This video delivers a detailed explanation of a complex S10 segmentectomy performed via uniportal incision. The surgeons utilize ICG technology to demarcate the intersegmental plane—a key step in achieving adequate resection margins and allowing a degree of necessary parenchymal sparing. The video also clearly illustrates the subsegmental bronchial and vascular anatomy, as well as the required instruments and energy device for this highly complex segment resection with minimal air leak.